cellulase free xylanase by thermomyces

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Optimization of the Cellulase Free Xylanase Production by

xylanase production by the immobilized cells of Bacillus pumilus was higher by 40.5 and 132.6 % over the free cells respectively after 48 and 72 h of the incubation. Keywords: Bacillus pumilus, Cellulase free xylanase, Calcium alginate, Immobilization

Purification and Characterization of Thermostable Cellulase Free Xylanase

Extracellular cellulase free xylanase from Pseudomonas sp. XPB-6 was purified to homogeneity with 2.15 fold of pu-rity and 60.31% yield, respectively by ammonium sulfate precipitation and DEAE ion exchange chromatography. The

A novel cellulase free alkaliphilic xylanase from alkali tolerant

A novel cellulase free alkaliphilic xylanase from alkali tolerant Penicillium citrinum: production, puriﬁcation and characterization T. Dutta, R. Sengupta, R. Sahoo, S. Sinha Ray, A. Bhattacharjee and S. Ghosh Department of Biochemistry, University College of

(PDF) A novel thermostable cellulase free xylanase stable in

A novel thermostable cellulase free xylanase stable in broad range of pH from Streptomyces sp. CS428 Process Biochemistry, 2013 Pradeep G.C. Yoon Seok Choi Seung Cho Hyo Lee Chi Seong

Production of high level of cellulase

Production of high level of cellulase-free and thermostable xylanase by a wild strain of Thermomyces lanuginosus Joseph Gomes, Isidore Gomes, W. Kreiner, H. Esterbauer, M. Sinner, Walter Steiner Institute of Biotechnology and Biochemical Engineering (6510)

Applied Sciences

Cellulase and xylanase production was optimal at the following conditions: pH 6 and 8, incubation temperature of 40 and 35 C, and agitation speed of 100 and 150 rpm, respectively. Under optimum conditions, 0.26 U/mL and 18.54 U/mL activities were attained at 60 and 48 h with specific productivity of 205 and 7417 U/g for cellulase and xylanase, respectively.

Bioflocculants' production from a cellulase

Cellulase-free xylanase Pseudomonas boreopolis G22 Biomass Bioﬂocculant Microalgae harvest ABSTRACT The major problem for industrial application of bioﬂocculants is its high production cost. Here, a novel bac-terium Pseudomonas boreopolis G22, which

Optimization of Cellulase

2014/5/22Cellulase-free xylanase production by thermophilic Streptomyces thermovulgaris TISTR1948 was cultivated in a basal medium with rice straw as sole source of carbon and as an inducible substrate. Variable medium components were selected in accordance with the Plackett-Burman experimental design.

African Journal of Biotechnology

2015/6/10Xylanases have largely been obtained from filamentous fungi and bacteria; few studies have investigated the production of this enzyme by yeasts. The aim of this study was to isolate yeasts from different sources, such as vegetables, cereal grains, fruits, and agro-industrial waste and to obtain yeasts capable of producing celulase-free xylanase. Samples were enriched using yeast malt broth

Production of high level of cellulase

TY - JOUR T1 - Production of high level of cellulase-free xylanase by the thermophilic fungus Thermomyces lanuginosus AU - Gomes, Joseph AU - Purkarthofer, H. AU - Hayn, M. AU - Sinner, M. AU - Steiner, Walter PY - 1993 Y1 - 1993 U2 - 10.1007

A Review of Xylanase Production by the Fermentation of Xylan:

2013/5/15[] used different preparations of commercial Xylanase P and crude xylanase from Thermomyces lanuginosus to evaluate the bleaching process of paper pulp. It was demonstrated that the use of enzymes could increase the pulp brightness (over 5 brightness points over the control) and reduce the amount of bleaching chemicals used (up to 30% for chlorine dioxide).

African Journal of Biotechnology

2015/6/10Xylanases have largely been obtained from filamentous fungi and bacteria; few studies have investigated the production of this enzyme by yeasts. The aim of this study was to isolate yeasts from different sources, such as vegetables, cereal grains, fruits, and agro-industrial waste and to obtain yeasts capable of producing celulase-free xylanase. Samples were

Isolation, identification and screening of potential cellulase

cellulase-free xylanase producing fungi Boddireddy Sridevi1* and M. A. Singara Charya2 1Department of Microbiology, Chaitanya Degree and PG College, (Autonomous), Warangal-506 001, Andhra Pradesh, India. 2Department of Microbiology, Kakatiya In order

Bioprocess and biotechnology: effect of xylanase from Aspergillus

2013/8/13Cellulose-free, alkali and thermo-stable microbial xylanases are mostly ideal for biopulping and bleaching processes (Birijlall et al., 2011; Yeasmin et al., 2011; Peixoto-Nogueira et al., 2009). This paper shows an important comparing of the xylanase production by A. niger and A. flavus using agroindustrial residues as carbon sources and tested their suitability for

International Research Journal of Biological Sciences : Production of cellulase free thermostable xylanase

Poorma C.A. and Prema P., Production of cellulase free endoxylanase from novel alkalophilic thermotolerant Bacillus pumilus by solid state fermentation and its application in waste paper recycling, Biores.Technol., 98, 485-490 (2007)

Optimization of Cellulase

2011/3/7Cellulase-free xylanase production by thermophilic Streptomyces thermovulgaris TISTR1948 was cultivated in a basal medium with rice straw as sole source of carbon and as an inducible substrate. Variable medium components were selected in accordance with the Plackett-Burman experimental design.

Sustainability

2018/7/12Again, mixing did not improve cellulase and xylanase production, obtaining xylanase peak at 90 h with a slightly lower value of 21 8 UA g −1 DM in comparison with the static fermentation. These results are in accordance with those reported by Deschamps et al. [ 34 ], where a reduction of cellulase activity was observed between static and mixed SSF, decreasing from 18 to 11 FPU per gram of

(PDF) Rapid production of thermostable cellulase

Enzyme Microb Tech- Another property of this xylanase is to be cellulase-free, nol 1992;14:272– 6. being a good candidate to further test its applicability in [18] Viikari L, Kantelinen A, Buchert J, Puls J. Enzymatic accessibility of

Biochemical and catalytic properties of endo

Endoxylanases from the thermophilic fungus, Thermomyces lanuginosus ATCC 44008 (cellulase free wild and mutant strains), were purified to homogeneity by anion-exchange and molecular-sieve chromatographic methods. The purified enzymes were monomers with molecular masses of 22 kDa (wild type) and 24 kDa (mutant), estimated by SDS-PAGE and gel filtration. As glycoproteins, the purified

Optimization of upstream and downstream process parameters for cellulase

2015/2/11Background Xylanases are important members of the hemicellulolytic enzyme system. Xylanase plays a vital role in the hydrolysis of major hemicellulosic component xylan and converts it into xylooligosaccharides and ultimately yields xylose. Cellulase-lacking or cellulase-poor xylanase with high temperature and pH stability has gained special attention, especially

Optimization, Isolation and Characterization of Cellulase–Free Thermostable Xylanase

cellulase free xylanase. The enzyme secretion was enhanced when the medium was supplemented with 0.5% wheat bran xylan, peptone and Ca2+ salt. The peak in xylanase production was achieved within 48-60 hours at temperature 50 -55 C and at pH 7.0.The

Production of cellulase

Production of cellulase-free xylanase by Aspergillus flavus: Effect of polyols on the thermostability and its application on cellulose pulp biobleaching Patricia Oliveira da Silva1, Nelciele Cavalieri de Alencar Guimares1, Simone de Carvalho Peixoto-Nogueira221

(PDF) A novel thermostable cellulase free xylanase stable in

A novel thermostable cellulase free xylanase stable in broad range of pH from Streptomyces sp. CS428 Process Biochemistry, 2013 Pradeep G.C. Yoon Seok Choi

OPTIMIZATION OF BIOSYNTHESIS CONDITIONS AND CATALITIC BEHAVIOR EVALUATION OF CELLULASE

Cellulase-free xylanase by Streptomyces sp.P12-137 was obtained by cultivation on the wheat bran as the sole carbon source. The effect of carbon and nitrogen sources and a ratio of them on the cellulase-free xylanase production was investigated. The new

Purification and Characterization of Thermostable Cellulase Free Xylanase

Extracellular cellulase free xylanase from Pseudomonas sp. XPB-6 was purified to homogeneity with 2.15 fold of purity and 60.31% yield, respectively by ammonium sulfate precipitation and DEAE ion exchange chromatography. The protein was found to be a homotrimer consisting of three subunits of 41 kDa each and the native molecular weight of 123 kDa.

Optimization of Cellulase

Cellulase-free xylanase production by thermophilic Streptomyces thermovulgaris TISTR1948 was cultivated in a basal medium with rice straw as sole source of carbon and as an inducible substrate. Variable medium components were selected in accordance with

Production of a cellulase

Indian Journal of Experimental Biology Vol. 53, June 2015, pp. 356-363 Production of a cellulase-free alkaline xylanase from Bacillus pumilus MTCC 5015 by submerged fermentation and its application in biobleaching Leya Thomas*, Raveendran Sindhu

Applied Sciences

2016/10/9Cellulase and xylanase are in high demand for application in several industrial processes, consequently necessitating the bioprospecting and manipulation of microbes for novel and greater enzyme productivity. This study reports on the optimal conditions for cellulase and xylanase production by a marine bacterial isolate from Nahoon beach sediment, via the

Optimization of cellulase

isolated from mushroom compost, produced a cellulase-free extracellular endo-1, 4-β-xylanase (EC 3.2.1.8) at 35 C and pH 8.0. Apple pomace—an inexpensive and abundant source of carbon—supported maximal xylanase activity of 500.10 U/g dry bacterial

Amazon: Characterization of a cellulase

Characterization of a cellulase-free, neutral xylanase from Thermomyces lanuginosus CBS 288.54 and its biobleaching effect on wheat straw pulp [An article from: Bioresource Technology] by X.T. Li (Author), Z.Q. Jiang (Author), L.T. Li (Author), S.Q. Yang (Author), W.Y Feng (Author) 2 more Format: Digital

The growth of Thermomyces lanuginosus (TSIKL.) isolates from

The lipolytic activity of Thermomyces lanuginosus strains isolated from different natural sources, International Biodeterioration Biodegradation, 55, 149-152 (2005). [15] Jatinder K., Chadha B.S., Saini H.S.: Optimization of culture conditions for production of cellulasesand xylanases by Scytalidium thermophilum using response surface methodology, World Journal of Microbiology and

Optimization of the Cellulase Free Xylanase Production by

2018/12/12Conclusion: Cellulase free xylanase production from the immobilized Bacillus pumilus IMAU80221 was optimized. The xylanase production by the immobilized cells of Bacillus pumilus was higher by 40.5 and 132.6 % over the free cells respectively after 48 and 72 h of the incubation.

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